Hotspot

trnS-GCU-ycf12

LSC · 6737-7294 · 558 bp

Back to Lemmaphyllum

Pi 0.00539

MarkerSeek score 61.10

Primer available yes

Label trnS-GCU-ycf12

Feature Metadata

label_name: trnS-GCU-ycf12
feature_type: igs
region: LSC
length_bp: 558
variable_sites: 3
pi: 0.00539
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-ycf12_p1	GGGAGAGAGAGGGATTCGAA	AACGGTCCTGATGCAACTAC	55.0	50.0	57.9	57.9	707-708 bp	1.0	79.1
trnS-GCU-ycf12_p2	GGGAGAGAGAGGGATTCGAA	ACTAACGGTCCTGATGCAAC	55.0	50.0	57.9	57.9	710-711 bp	1.0	79.1
trnS-GCU-ycf12_p3	AAGGGAGAGAGAGGGATTCG	AACGGTCCTGATGCAACTAC	55.0	50.0	57.9	57.9	709-710 bp	1.0	79.0
trnS-GCU-ycf12_p4	AAGGGAGAGAGAGGGATTCG	ACTAACGGTCCTGATGCAAC	55.0	50.0	57.9	57.9	712-713 bp	1.0	79.0
trnS-GCU-ycf12_p5	GCAATCCGACGCTTTAAACC	AACGGTCCTGATGCAACTAC	50.0	50.0	58.1	57.9	655-656 bp	1.0	78.9