Hotspot

matK-rps16

LSC · 1759-2406 · 648 bp

Back to Lemmaphyllum

Pi 0.00154

MarkerSeek score 59.60

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 648
variable_sites: 1
pi: 0.00154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	TGCATCAAATCTAAGAGAGGACC	TATCTCGAAAGGGGCAAGGT	43.5	50.0	58.0	58.4	754 bp	1.0	52.5
matK-rps16_p2	GAGGACCCATAAGTTATTCTCGT	TATCTCGAAAGGGGCAAGGT	43.5	50.0	57.5	58.4	738 bp	1.0	48.5
matK-rps16_p3	TGCATCAAATCTAAGAGAGGACC	ATATCTCGAAAGGGGCAAGG	43.5	50.0	58.0	57.1	755 bp	1.0	48.4
matK-rps16_p4	TCAAATCTAAGAGAGGACCCA	TATCTCGAAAGGGGCAAGGT	42.9	50.0	55.4	58.4	750 bp	1.0	48.0
matK-rps16_p5	GCATCAAATCTAAGAGAGGACCC	TATCTCGAAAGGGGCAAGGT	47.8	50.0	58.6	58.4	753 bp	1.0	47.8