Pi
0.00169
MarkerSeek score
52.70
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | TGGAAAGTTGCAAAATCATCA | TATAGGTGGCAGTCGCATCT | 33.3 | 50.0 | 54.5 | 58.3 | 1162 bp | 1.0 | 42.1 |
| ycf1_p2 | TGGAAAGTTGCAAAATCATCA | GGCAGTCGCATCTCTTCTTA | 33.3 | 50.0 | 54.5 | 57.4 | 1155 bp | 1.0 | 40.1 |
| ycf1_p3 | ATGGAAAGTTGCAAAATCATCA | TATAGGTGGCAGTCGCATCT | 31.8 | 50.0 | 55.1 | 58.3 | 1163 bp | 1.0 | 40.1 |
| ycf1_p4 | TGGAAAGTTGCAAAATCATCAT | TATAGGTGGCAGTCGCATCT | 31.8 | 50.0 | 55.1 | 58.3 | 1162 bp | 1.0 | 40.1 |
| ycf1_p5 | TGGAAAGTTGCAAAATCATCA | ATATAGGTGGCAGTCGCATC | 33.3 | 50.0 | 54.5 | 57.2 | 1163 bp | 1.0 | 40.1 |
| ycf1_p1 | AATTGGTATGACCCACGGTT | TGGGGGAAATCCAGCATAGA | 45.0 | 50.0 | 57.1 | 58.1 | 314 bp | 1.0 | 72.6 |
| ycf1_p2 | ATTGGTATGACCCACGGTTT | TGGGGGAAATCCAGCATAGA | 45.0 | 50.0 | 57.1 | 58.1 | 313 bp | 1.0 | 72.6 |
| ycf1_p3 | AATTGGTATGACCCACGGTT | TATTCGGGGTATGGGGGAAA | 45.0 | 50.0 | 57.1 | 57.8 | 325 bp | 1.0 | 71.7 |
| ycf1_p4 | ATTGGTATGACCCACGGTTT | TATTCGGGGTATGGGGGAAA | 45.0 | 50.0 | 57.1 | 57.8 | 324 bp | 1.0 | 71.7 |
| ycf1_p5 | AATTGGTATGACCCACGGTTT | TGGGGGAAATCCAGCATAGA | 42.9 | 50.0 | 57.8 | 58.1 | 314 bp | 1.0 | 70.4 |