Hotspot

rpoC2

LSC · 15818-19939 · 4122 bp

Back to Leiospora

Pi 0.00121

MarkerSeek score 49.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4122
variable_sites: 5
pi: 0.00121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTGTGGACGTTTCCTCACAA	CCCCTTAGGAACAGCCATTC	45.0	55.0	57.6	57.9	269 bp	1.0	76.9
rpoC2_p2	TTTGTGGACGTTTCCTCACA	CCCCTTAGGAACAGCCATTC	45.0	55.0	57.6	57.9	270 bp	1.0	76.9
rpoC2_p3	TGTGGACGTTTCCTCACAAT	CCCCTTAGGAACAGCCATTC	45.0	55.0	57.4	57.9	268 bp	1.0	75.1
rpoC2_p4	TTGTGGACGTTTCCTCACAA	ATTAAAAACTCGTCGGGCCC	45.0	50.0	57.6	58.5	286 bp	1.0	73.0
rpoC2_p5	TTTGTGGACGTTTCCTCACA	ATTAAAAACTCGTCGGGCCC	45.0	50.0	57.6	58.5	287 bp	1.0	73.0