Hotspot

ndhF-rpl32

SSC · 112667-113515 · 849 bp

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Pi 0.00236
MarkerSeek score 60.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
849
variable_sites
2
pi
0.00236
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCTGTCGGAAAAAGT ACGATGTCCAATATCCCTTCC 40.0 47.6 56.4 57.2 1034 bp 1.0 53.2
ndhF-rpl32_p2 TTTGTTGCTGTCGGAAAAAGT ACGATGTCCAATATCCCTTCC 38.1 47.6 57.1 57.2 1035 bp 1.0 51.2
ndhF-rpl32_p3 TTGTTGCTGTCGGAAAAAGT CGATGTCCAATATCCCTTCCT 40.0 47.6 56.4 56.9 1033 bp 1.0 50.9
ndhF-rpl32_p4 TTGTTGCTGTCGGAAAAAGT AACGATGTCCAATATCCCTTCC 40.0 45.5 56.4 57.9 1035 bp 1.0 50.7
ndhF-rpl32_p5 TTGTTGCTGTCGGAAAAAGT AAGCTTTCAACGATGTCCAA 40.0 40.0 56.4 55.9 1043 bp 1.0 50.4