Hotspot

psaJ-rpl33

LSC · 66794-67208 · 415 bp

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Pi 0.00242

MarkerSeek score 47.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 415
variable_sites: 1
pi: 0.00242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TGGTTCGTTTCATTAGCAGGT	ATATCTGGAAATTCCCGCCG	42.9	50.0	57.9	57.8	592 bp	1.0	69.7
psaJ-rpl33_p2	TGGTTCGTTTCATTAGCAGGT	AATTCCCGCCGATTCTTTCT	42.9	45.0	57.9	57.6	583 bp	1.0	67.8
psaJ-rpl33_p3	TGGTTCGTTTCATTAGCAGGT	ATTCCCGCCGATTCTTTCTT	42.9	45.0	57.9	57.6	582 bp	1.0	67.8
psaJ-rpl33_p4	TGGTTCGTTTCATTAGCAGGT	GGAAATTCCCGCCGATTCTT	42.9	50.0	57.9	58.6	586 bp	1.0	66.3
psaJ-rpl33_p5	TGGTTCGTTTCATTAGCAGGT	TATCTGGAAATTCCCGCCGA	42.9	50.0	57.9	58.9	591 bp	1.0	64.2