Hotspot

psbE-petL

LSC · 64059-65437 · 1379 bp

Back to Leiospora

Pi 0.00219

MarkerSeek score 60.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1379
variable_sites: 3
pi: 0.00219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	57.9	56.3	1524-1530 bp	1.0	66.3
psbE-petL_p2	AATAACCAGCCCGCAATGAA	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1525-1531 bp	1.0	65.6
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1442-1448 bp	1.0	65.6
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	1437-1443 bp	1.0	65.3
psbE-petL_p5	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGAA	45.0	42.9	57.9	57.0	1524-1530 bp	1.0	64.3