Hotspot

trnT-ACA-trnL-UUA

LSC · 45997-46769 · 773 bp

Back to Leiospora

Pi 0.0013
MarkerSeek score 59.60
Primer available yes
Label trnT-ACA-trnL-UUA

Feature Metadata

label_name
trnT-ACA-trnL-UUA
feature_type
igs
region
LSC
length_bp
773
variable_sites
1
pi
0.0013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-ACA-trnL-UUA_p1 GCGATGCTCTAACCTCTGAG CTACCGATTCCGCCATATCC 55.0 55.0 57.9 57.7 825-826 bp 1.0 76.8
trnT-ACA-trnL-UUA_p2 GGAATCGAACCGATGACCAT CTACCGATTCCGCCATATCC 50.0 55.0 57.8 57.7 857-858 bp 1.0 75.9
trnT-ACA-trnL-UUA_p3 GCGATGCTCTAACCTCTGAG GGTTTCCATACCAAGGCTCA 55.0 50.0 57.9 57.5 866-867 bp 1.0 75.1
trnT-ACA-trnL-UUA_p4 GGAATCGAACCGATGACCAT GGTTTCCATACCAAGGCTCA 50.0 50.0 57.8 57.5 898-899 bp 1.0 74.2
trnT-ACA-trnL-UUA_p5 CTAACCTCTGAGCTAAGCGG CTACCGATTCCGCCATATCC 55.0 55.0 57.5 57.7 817-818 bp 1.0 73.8