Hotspot

trnE-GAA-trnT-ACC

LSC · 30465-31095 · 631 bp

Back to Leiospora

Pi 0.00159
MarkerSeek score 60.00
Primer available yes
Label trnE-GAA-trnT-ACC

Feature Metadata

label_name
trnE-GAA-trnT-ACC
feature_type
igs
region
LSC
length_bp
631
variable_sites
1
pi
0.00159
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-GAA-trnT-ACC_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 718-720 bp 1.0 79.1
trnE-GAA-trnT-ACC_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 716-718 bp 1.0 79.1
trnE-GAA-trnT-ACC_p3 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 772-774 bp 1.0 79.0
trnE-GAA-trnT-ACC_p4 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 697-699 bp 1.0 78.7
trnE-GAA-trnT-ACC_p5 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 695-697 bp 1.0 78.7