Hotspot

trnH-CAC-psbA

LSC · 77-423 · 347 bp

Back to Leiospora

Pi 0.00593
MarkerSeek score 59.70
Primer available yes
Label trnH-CAC-psbA

Feature Metadata

label_name
trnH-CAC-psbA
feature_type
igs
region
LSC
length_bp
347
variable_sites
2
pi
0.00593
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-CAC-psbA_p1 CTGCCTTAATCCACTTGGCT GCTGTTGAGGCTCCATCTAC 50.0 55.0 57.9 58.1 395-405 bp 1.0 80.9
trnH-CAC-psbA_p2 CTGCCTTAATCCACTTGGCT GAGGCTCCATCTACAAACGG 50.0 55.0 57.9 58.1 389-399 bp 1.0 80.9
trnH-CAC-psbA_p3 ACTGCCTTAATCCACTTGGC GCTGTTGAGGCTCCATCTAC 50.0 55.0 58.2 58.1 396-406 bp 1.0 80.7
trnH-CAC-psbA_p4 ACTGCCTTAATCCACTTGGC GAGGCTCCATCTACAAACGG 50.0 55.0 58.2 58.1 390-400 bp 1.0 80.7
trnH-CAC-psbA_p5 AATCCACTTGGCTACATCCG GCTGTTGAGGCTCCATCTAC 50.0 55.0 57.7 58.1 388-398 bp 1.0 79.4