Hotspot

rps15

SSC · 120947-121225 · 279 bp

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Pi 0.454

MarkerSeek score 76.10

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 279
variable_sites: 114
pi: 0.454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TGAGGTAAATCAGCAGCGAC	CGCTGTCTTACATCCCAGTC	50.0	55.0	58.0	58.1	1159 bp	0.5	59.3
rps15_p2	GAAAGGAGTCTGTGCACCAA	CGCTGTCTTACATCCCAGTC	50.0	55.0	58.0	58.1	1046 bp	0.5	59.2
rps15_p3	TCTGTGCACCAATATCTGCC	CGCTGTCTTACATCCCAGTC	50.0	55.0	58.0	58.1	1038 bp	0.5	59.1
rps15_p4	TGAGGTAAATCAGCAGCGAC	GGGAGCCGTTTGAGTATGTT	50.0	50.0	58.0	57.9	1319 bp	0.5	59.0
rps15_p5	AACACCATGCATTGATGGGT	CGCTGTCTTACATCCCAGTC	45.0	55.0	58.1	58.1	728 bp	0.5	59.0