Hotspot

ndhI

SSC · 116956-117456 · 501 bp

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Pi 0.487

MarkerSeek score 80.50

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 501
variable_sites: 223
pi: 0.487
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AGACCACGGAAGTTACTCCA	AGCCGAGTGTGAACGATTAC	50.0	50.0	58.0	58.0	1664 bp	0.5	59.9
ndhI_p2	TAATACTACTCCCAGGCCCG	AGCCGAGTGTGAACGATTAC	55.0	50.0	58.0	58.0	1438 bp	0.5	59.9
ndhI_p3	AGACTTGTACAGACCACGGA	AGCCGAGTGTGAACGATTAC	50.0	50.0	58.0	58.0	1674 bp	0.5	59.9
ndhI_p4	AGACCACGGAAGTTACTCCA	TTTGTGACCTTTGAGGAGCC	50.0	50.0	58.0	58.0	1870 bp	0.5	59.8
ndhI_p5	TAATACTACTCCCAGGCCCG	TTTGTGACCTTTGAGGAGCC	55.0	50.0	58.0	58.0	1644 bp	0.5	59.8