Hotspot

ndhH

SSC · 119674-120855 · 1182 bp

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Pi 0.473

MarkerSeek score 82.40

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 507
pi: 0.473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TGCGTATTCAGGGCCAATAC	CGCTGTCTTACATCCCAGTC	50.0	55.0	58.0	58.1	2037 bp	0.5	59.1
ndhH_p2	GTTGTTGTATCCCCGCAGAT	CGCTGTCTTACATCCCAGTC	50.0	55.0	58.0	58.1	2017 bp	0.5	59.1
ndhH_p3	ATACGTTGTTGTATCCCCGC	CGCTGTCTTACATCCCAGTC	50.0	55.0	58.1	58.1	2021 bp	0.5	59.0
ndhH_p4	CCTGACATAAGAAGGCCGAC	CGCTGTCTTACATCCCAGTC	55.0	55.0	58.1	58.1	2282 bp	0.5	59.0
ndhH_p5	GACATAAGAAGGCCGACAGG	CGCTGTCTTACATCCCAGTC	55.0	55.0	58.1	58.1	2279 bp	0.5	59.0