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ndhG

SSC · 116074-116604 · 531 bp

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Pi 0.48

MarkerSeek score 78.40

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 208
pi: 0.48
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CGATGAGTTCCCCGAGTTTT	TCAAATTGCTTTGGGTCGGT	50.0	45.0	57.9	58.3	1648 bp	0.5	56.8
ndhG_p2	GAATCGATGAGTTCCCCGAG	TCAAATTGCTTTGGGTCGGT	55.0	45.0	57.9	58.3	1652 bp	0.5	56.5
ndhG_p3	CTTCTGGATTGACAGTGCCA	TCAAATTGCTTTGGGTCGGT	50.0	45.0	57.8	58.3	1616 bp	0.5	56.1
ndhG_p4	ACTTCTGGATTGACAGTGCC	TCAAATTGCTTTGGGTCGGT	50.0	45.0	57.8	58.3	1617 bp	0.5	56.1
ndhG_p5	CGATGAGTTCCCCGAGTTTT	TAGGAGGCGAGAATGGTGTT	50.0	50.0	57.9	58.4	1480 bp	0.5	55.8