Hotspot

ccsA

SSC · 112226-113194 · 969 bp

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Pi 0.498

MarkerSeek score 81.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 425
pi: 0.498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TAGACACGCTGCTCTTAGGA	AGAACCGCGTGAATCACTAC	50.0	50.0	57.9	58.0	1236 bp	0.5	58.9
ccsA_p2	AAATTGGTAGACACGCTGCT	AGAACCGCGTGAATCACTAC	45.0	50.0	57.8	58.0	1243 bp	0.5	58.5
ccsA_p3	GGTAGACACGCTGCTCTTAG	AGAACCGCGTGAATCACTAC	55.0	50.0	57.8	58.0	1238 bp	0.5	58.4
ccsA_p4	GTAGACACGCTGCTCTTAGG	AGAACCGCGTGAATCACTAC	55.0	50.0	57.8	58.0	1237 bp	0.5	58.4
ccsA_p5	TAGACACGCTGCTCTTAGGA	GCATTACCGGGTATGAGTGG	50.0	55.0	57.9	58.1	1725 bp	0.5	58.0