Hotspot

rps15-ycf1

SSC · 121226-121626 · 401 bp

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Pi 0.483

MarkerSeek score 77.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 401
variable_sites: 170
pi: 0.483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCGCAGACCTCTCTGAGATA	GTCTCCTGGCTAGCCTGATA	55.0	55.0	58.0	58.0	1216 bp	0.5	59.8
rps15-ycf1_p2	CCGCAGACCTCTCTGAGATA	ATAGTCTCCTGGCTAGCCTG	55.0	55.0	58.0	58.0	1219 bp	0.5	59.7
rps15-ycf1_p3	AACACCATGCATTGATGGGT	GTCTCCTGGCTAGCCTGATA	45.0	55.0	58.1	58.0	1493 bp	0.5	59.5
rps15-ycf1_p4	AACACCATGCATTGATGGGT	ATAGTCTCCTGGCTAGCCTG	45.0	55.0	58.1	58.0	1496 bp	0.5	59.5
rps15-ycf1_p5	CCGCAGACCTCTCTGAGATA	AATTATGGAACCTGCCCGTC	55.0	50.0	58.0	57.9	1092 bp	0.5	59.4