Hotspot

ndhG-ndhI

SSC · 116605-116955 · 351 bp

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Pi 0.455

MarkerSeek score 76.50

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 351
variable_sites: 141
pi: 0.455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AGACCACGGAAGTTACTCCA	GATGGACTTTACCGAGGCTG	50.0	55.0	58.0	58.1	1359 bp	0.5	59.5
ndhG-ndhI_p2	TAATACTACTCCCAGGCCCG	GATGGACTTTACCGAGGCTG	55.0	55.0	58.0	58.1	1133 bp	0.5	59.5
ndhG-ndhI_p3	AGACTTGTACAGACCACGGA	GATGGACTTTACCGAGGCTG	50.0	55.0	58.0	58.1	1369 bp	0.5	59.5
ndhG-ndhI_p4	TCCCAGGCCCGCTAATATAA	GATGGACTTTACCGAGGCTG	50.0	55.0	58.0	58.1	1124 bp	0.5	59.3
ndhG-ndhI_p5	TAATACTACTCCCAGGCCCG	AGATGGACTTTACCGAGGCT	55.0	50.0	58.0	58.1	1134 bp	0.5	58.9