Hotspot

trnL-UAG-ccsA

SSC · 112129-112225 · 97 bp

Back to Leibnitzia

Pi 0.478

MarkerSeek score 77.30

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 97
variable_sites: 44
pi: 0.478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GAAAGGATATTGGTCGGCGT	TGATCCACAAAGAAGAGCCG	50.0	50.0	58.1	57.9	1267 bp	0.5	58.8
trnL-UAG-ccsA_p2	TAGACACGCTGCTCTTAGGA	TGATCCACAAAGAAGAGCCG	50.0	50.0	57.9	57.9	620 bp	0.5	58.2
trnL-UAG-ccsA_p3	GAAAGGATATTGGTCGGCGT	TAGCCCATACTGCTCCTGAA	50.0	50.0	58.1	57.8	1541 bp	0.5	58.2
trnL-UAG-ccsA_p4	GAAAGGATATTGGTCGGCGT	TTAGCCCATACTGCTCCTGA	50.0	50.0	58.1	57.8	1542 bp	0.5	58.2
trnL-UAG-ccsA_p5	AAATTGGTAGACACGCTGCT	TGATCCACAAAGAAGAGCCG	45.0	50.0	57.8	57.9	627 bp	0.5	57.7