Hotspot

trnM-CAU-atpE

LSC · 51590-51815 · 226 bp

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Pi 0.0398

MarkerSeek score 75.40

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 226
variable_sites: 9
pi: 0.0398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	CAAATCTAGCTCTCCGACGG	50.0	55.0	58.4	57.9	334-348 bp	1.0	77.8
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	GGCAAATCTAGCTCTCCGAC	50.0	55.0	58.4	58.1	336-350 bp	1.0	77.7
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	GAGGCAAATCTAGCTCTCCG	50.0	55.0	58.4	57.9	338-352 bp	1.0	77.6
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	AGGCAAATCTAGCTCTCCGA	50.0	50.0	58.4	58.2	337-351 bp	1.0	77.1
trnM-CAU-atpE_p5	TATTGCTTTCATACGGCGGG	CTAGGACACGAGTAGAGGCT	50.0	55.0	58.4	57.7	313-327 bp	1.0	76.3