Hotspot

ycf3

LSC · 42431-44411 · 1981 bp

Back to Leersia

Pi 0.00454

MarkerSeek score 46.00

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1981
variable_sites: 9
pi: 0.00454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	GGGGCCCTTAGCTATTGTTC	TTGAGCTGAGCGGAAATACC	55.0	50.0	58.0	58.0	2725-2730 bp	1.0	80.0
ycf3_p2	GCATCAGCATGTAGGTTCCA	TTGAGCTGAGCGGAAATACC	50.0	50.0	58.0	58.0	2763-2768 bp	1.0	79.8
ycf3_p3	AAAGAGACCCGCCAACTTTT	TTGAGCTGAGCGGAAATACC	45.0	50.0	57.9	58.0	2575-2580 bp	1.0	79.6
ycf3_p4	AAGAGACCCGCCAACTTTTT	TTGAGCTGAGCGGAAATACC	45.0	50.0	57.9	58.0	2574-2579 bp	1.0	79.6
ycf3_p5	GTCTGGCCCATTCCTCAAAA	TTGAGCTGAGCGGAAATACC	50.0	50.0	58.1	58.0	2690-2695 bp	1.0	79.5