Hotspot

psbA

LSC · 82-1143 · 1062 bp

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Pi 0

MarkerSeek score 29.40

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGCTATATGTTAGCAGAAAAGCC	AATACGTGTGCTTGGGAGTC	39.1	50.0	57.0	57.9	1135 bp	1.0	47.2
psbA_p2	TGCTATATGTTAGCAGAAAAGCC	TGGGAGTCCTTGCAATTTGA	39.1	45.0	57.0	57.3	1123 bp	1.0	42.5
psbA_p3	TGCTATATGTTAGCAGAAAAGCC	GCTTGGGAGTCCTTGCAATT	39.1	50.0	57.0	58.7	1126 bp	1.0	42.0
psbA_p4	GCTATATGTTAGCAGAAAAGCC	AATACGTGTGCTTGGGAGTC	40.9	50.0	55.3	57.9	1134 bp	1.0	41.8
psbA_p5	TGGGTATTTCTAGCTTTACTTCCT	AATACGTGTGCTTGGGAGTC	37.5	50.0	57.1	57.9	1167 bp	1.0	40.2