Hotspot

ndhG

SSC · 109356-109886 · 531 bp

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Pi 0.00565

MarkerSeek score 47.10

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 3
pi: 0.00565
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACCATGTTTCGGCTTGTGAT	CAGCGAGGTACATTGGTCAA	45.0	50.0	58.1	57.9	1557-1563 bp	1.0	79.2
ndhG_p2	ACCATGTTTCGGCTTGTGAT	TGTGAAGTATGTGTTCGCGT	45.0	45.0	58.1	57.9	1411-1417 bp	1.0	78.9
ndhG_p3	AGCTCTAACCATGTTTCGGC	CAGCGAGGTACATTGGTCAA	50.0	50.0	58.3	57.9	1564-1570 bp	1.0	77.8
ndhG_p4	GCTCTAACCATGTTTCGGCT	CAGCGAGGTACATTGGTCAA	50.0	50.0	58.3	57.9	1563-1569 bp	1.0	77.8
ndhG_p5	GCTCTAACCATGTTTCGGCT	TGTGAAGTATGTGTTCGCGT	50.0	45.0	58.3	57.9	1417-1423 bp	1.0	77.5