Hotspot

ndhF-rpl32

SSC · 103679-104513 · 835 bp

Back to Leersia

Pi 0.00524

MarkerSeek score 62.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 835
variable_sites: 4
pi: 0.00524
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGAAGTGGGAGAAAAGGGA	TCCAAAGATTTTTACGAATACGCT	50.0	33.3	58.2	57.3	877-947 bp	1.0	45.1
ndhF-rpl32_p2	CTGGAAGTGGGAGAAAAGGG	TCCAAAGATTTTTACGAATACGCT	55.0	33.3	57.8	57.3	878-948 bp	1.0	44.9
ndhF-rpl32_p3	TGGAAGTGGGAGAAAAGGGA	TGACATCGAAGTACGTTTTT	50.0	35.0	58.2	53.2	849-919 bp	1.0	44.9
ndhF-rpl32_p4	TGGAAGTGGGAGAAAAGGGA	TTGACATCGAAGTACGTTTT	50.0	35.0	58.2	53.2	850-920 bp	1.0	44.8
ndhF-rpl32_p5	TGGAAGTGGGAGAAAAGGGA	TTTGACATCGAAGTACGTTT	50.0	35.0	58.2	53.2	851-921 bp	1.0	44.8