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rps12-clpP

LSC · 68283-68416 · 134 bp

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Pi 0.0149

MarkerSeek score 61.10

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 134
variable_sites: 2
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CACCCTAGCACATGTTCCTC	GATTGACGAGCACTGCGATA	55.0	50.0	58.0	58.2	306 bp	1.0	79.0
rps12-clpP_p2	CCTAGCACATGTTCCTCGAC	GATTGACGAGCACTGCGATA	55.0	50.0	58.1	58.2	303 bp	1.0	78.7
rps12-clpP_p3	TGGCTGTCTTGCGTTTCTAA	GATTGACGAGCACTGCGATA	45.0	50.0	57.8	58.2	237 bp	1.0	77.4
rps12-clpP_p4	TTGGCTGTCTTGCGTTTCTA	GATTGACGAGCACTGCGATA	45.0	50.0	57.8	58.2	238 bp	1.0	77.4
rps12-clpP_p5	CACCCTAGCACATGTTCCTC	ATTGACGAGCACTGCGATAC	55.0	50.0	58.0	58.4	305 bp	1.0	76.8