Hotspot

psbE-petL

LSC · 63033-64275 · 1243 bp

Back to Leersia

Pi 0.00322

MarkerSeek score 60.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1243
variable_sites: 4
pi: 0.00322
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGCAGCCAATAGAAAACCGA	45.0	45.0	58.0	57.5	1400 bp	1.0	76.2
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.9	57.5	1389 bp	1.0	76.0
psbE-petL_p3	ACCAACCCGCAATGAATAGG	AGCAGCCAATAGAAAACCGA	50.0	45.0	58.2	57.5	1391 bp	1.0	74.5
psbE-petL_p4	ACCCGCAATGAATAGGGAAG	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.7	57.5	1387 bp	1.0	73.6
psbE-petL_p5	CCGCAATGAATAGGGAAGGT	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.7	57.5	1385 bp	1.0	73.6