Hotspot

petA-psbJ

LSC · 61296-62262 · 967 bp

Back to Leersia

Pi 0.00724

MarkerSeek score 59.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 7
pi: 0.00724
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	ACCGGTATTCCTGTGATTGG	50.0	50.0	57.9	57.3	1144-1147 bp	1.0	74.4
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	CCGGTATTCCTGTGATTGGT	50.0	50.0	57.9	57.3	1143-1146 bp	1.0	74.4
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	AACCGGTATTCCTGTGATTGG	50.0	47.6	57.9	58.0	1145-1148 bp	1.0	72.1
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	CCGGTATTCCTGTGATTGGTT	50.0	47.6	57.9	58.0	1143-1146 bp	1.0	72.1
petA-psbJ_p5	ATTACGCGTTCAAGGCCTTT	CCGGTATTCCTGTGATTGGT	45.0	50.0	58.5	57.3	1156-1159 bp	1.0	71.0