Hotspot

rbcL-accD

LSC · 56234-57265 · 1032 bp

Back to Leersia

Pi 0.0107

MarkerSeek score 50.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1032
variable_sites: 11
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TATACCGGTAGCTTCAGGGG	TGCGCCTATTTCGAATCCAA	55.0	45.0	58.0	58.0	1720-1721 bp	1.0	80.4
rbcL-accD_p2	TATACCGGTAGCTTCAGGGG	CCCTCAGCATACCGAAACTC	55.0	55.0	58.0	58.1	1602-1603 bp	1.0	80.2
rbcL-accD_p3	GAGCTGTATTTGCGAGGGAA	TGCGCCTATTTCGAATCCAA	50.0	45.0	58.0	58.0	2084-2085 bp	1.0	80.1
rbcL-accD_p4	AAGAGCTGTATTTGCGAGGG	TGCGCCTATTTCGAATCCAA	50.0	45.0	58.0	58.0	2086-2087 bp	1.0	80.1
rbcL-accD_p5	TATACCGGTAGCTTCAGGGG	TCGAATGCGCCTATTTCGAA	55.0	45.0	58.0	58.1	1725-1726 bp	1.0	80.1