Hotspot

trnG-UCC-trnfM-CAU

LSC · 11975-12391 · 417 bp

Back to Leersia

Pi 0.00785

MarkerSeek score 63.30

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 417
variable_sites: 3
pi: 0.00785
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	GCGAGCGTAGTTCAATGGTA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.1	57.8	500-535 bp	1.0	82.0
trnG-UCC-trnfM-CAU_p2	GCGAGCGTAGTTCAATGGTA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	58.1	58.3	515-550 bp	1.0	81.5
trnG-UCC-trnfM-CAU_p3	CGTAGCGAGCGTAGTTCAAT	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.2	57.8	504-539 bp	1.0	81.2
trnG-UCC-trnfM-CAU_p4	CCCAGGCGGATATACCCTAT	CGAGGCTCATAACCTTGAGG	55.0	55.0	57.8	57.8	535-570 bp	1.0	81.2
trnG-UCC-trnfM-CAU_p5	ATATAGGACGTAGCGAGCGT	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.2	57.8	512-547 bp	1.0	81.1