Hotspot

trnS-UGA-psbZ

LSC · 11114-11504 · 391 bp

Back to Leersia

Pi 0.00278

MarkerSeek score 60.00

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 391
variable_sites: 1
pi: 0.00278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	480-511 bp	1.0	82.4
trnS-UGA-psbZ_p2	GAGCTATCAACCACTCAGCC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	487-518 bp	1.0	82.1
trnS-UGA-psbZ_p3	GGAGCTATCAACCACTCAGC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	488-519 bp	1.0	82.1
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	495-526 bp	1.0	82.0
trnS-UGA-psbZ_p5	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	482-513 bp	1.0	81.9