Hotspot

trnS-GCU-psbD

LSC · 7443-8411 · 969 bp

Back to Leersia

Pi 0.0031

MarkerSeek score 60.60

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 969
variable_sites: 3
pi: 0.0031
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1113-1114 bp	1.0	78.6
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1100-1101 bp	1.0	78.0
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	AGGAAAAAGCAATAGGCCGG	45.0	50.0	58.0	58.5	1128-1129 bp	1.0	75.8
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	GGAAAAAGCAATAGGCCGGA	45.0	50.0	58.0	58.5	1127-1128 bp	1.0	75.7
trnS-GCU-psbD_p5	GCTTTAGTCCACTCAGCCAT	GCCGGACCATCCTACAAAAA	50.0	50.0	57.6	58.2	1099-1100 bp	1.0	75.6