Hotspot

trnN-GUU-ycf1

SSC · 51184-52062 · 879 bp

Back to Lecanorchis

Pi 0.13

MarkerSeek score 85.10

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: SSC
length_bp: 879
variable_sites: 102
pi: 0.13
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	CTCTACCGCTGAGCTACTGA	TAGCGCAACGACAGAATTGA	55.0	45.0	58.3	57.9	965-1007 bp	1.0	88.8
trnN-GUU-ycf1_p2	TCTACCGCTGAGCTACTGAG	TAGCGCAACGACAGAATTGA	55.0	45.0	58.3	57.9	964-1006 bp	1.0	88.8
trnN-GUU-ycf1_p3	CTCTACCGCTGAGCTACTGA	AATATAGCCCTAGCGCAACG	55.0	50.0	58.3	57.9	975-1017 bp	1.0	88.7
trnN-GUU-ycf1_p4	TCTACCGCTGAGCTACTGAG	AATATAGCCCTAGCGCAACG	55.0	50.0	58.3	57.9	974-1016 bp	1.0	88.7
trnN-GUU-ycf1_p5	TCTACCGCTGAGCTACTGAG	GCGCAACGACAGAATTGATT	55.0	45.0	58.3	57.8	962-1004 bp	1.0	87.4