Hotspot

rrn5-trnN-GUU

SSC · 49636-51111 · 1476 bp

Back to Lecanorchis

Pi 0.0834

MarkerSeek score 83.00

Primer available yes

Label rrn5-trnN-GUU

Feature Metadata

label_name: rrn5-trnN-GUU
feature_type: igs
region: SSC
length_bp: 1476
variable_sites: 116
pi: 0.0834
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnN-GUU_p1	AGGAGGGATCCTGTGGAAAA	AGGTCGTAGGTTCGAATCCT	50.0	50.0	57.9	57.9	1528-1544 bp	1.0	88.4
rrn5-trnN-GUU_p2	TGGTGTCCTAGGCATAGAGG	AGGTCGTAGGTTCGAATCCT	55.0	50.0	57.9	57.9	1606-1622 bp	1.0	87.9
rrn5-trnN-GUU_p3	AATCCATCCCGAACTTGGTG	AGGTCGTAGGTTCGAATCCT	50.0	50.0	57.9	57.9	1577-1593 bp	1.0	87.7
rrn5-trnN-GUU_p4	ACCAATCCATCCCGAACTTG	AGGTCGTAGGTTCGAATCCT	50.0	50.0	57.9	57.9	1580-1596 bp	1.0	87.7
rrn5-trnN-GUU_p5	CAATCCATCCCGAACTTGGT	AGGTCGTAGGTTCGAATCCT	50.0	50.0	57.9	57.9	1578-1594 bp	1.0	87.7