Hotspot

rrn16-rrn23

SSC · 44581-46342 · 1762 bp

Back to Lecanorchis

Pi 0.0882

MarkerSeek score 86.80

Primer available yes

Label rrn16-rrn23

Feature Metadata

label_name: rrn16-rrn23
feature_type: igs
region: SSC
length_bp: 1762
variable_sites: 146
pi: 0.0882
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rrn23_p1	CAAGGTAGCCGTACTGGAAG	ATTCGGGAATCTCCGGATCT	55.0	50.0	57.7	58.0	1918-2081 bp	1.0	87.3
rrn16-rrn23_p2	CAAGGTAGCCGTACTGGAAG	GTCTCTGGGTGCCTAGGTAT	55.0	55.0	57.7	57.9	1846-2009 bp	1.0	87.0
rrn16-rrn23_p3	AAGTCGTAACAAGGTAGCCG	ATTCGGGAATCTCCGGATCT	50.0	50.0	57.6	58.0	1927-2090 bp	1.0	86.8
rrn16-rrn23_p4	AACAAGGTAGCCGTACTGGA	ATTCGGGAATCTCCGGATCT	50.0	50.0	58.4	58.0	1920-2083 bp	1.0	86.8
rrn16-rrn23_p5	ACAAGGTAGCCGTACTGGAA	ATTCGGGAATCTCCGGATCT	50.0	50.0	58.4	58.0	1919-2082 bp	1.0	86.8