Hotspot

rpl2-rpl23

IRb · 28325-28828 · 504 bp

Back to Lecanorchis

Pi 0.0876

MarkerSeek score 78.70

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: IRb
length_bp: 504
variable_sites: 31
pi: 0.0876
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	ATTCCATTGCGTGTACTCGG	ATCCGTTATGGGACCAATGC	50.0	50.0	58.3	58.0	521-639 bp	1.0	85.3
rpl2-rpl23_p2	ACGGTGCTGTCCAAAAATCA	ATCCGTTATGGGACCAATGC	45.0	50.0	58.3	58.0	588-706 bp	1.0	85.2
rpl2-rpl23_p3	ACGATTCCATTGCGTGTACT	ATCCGTTATGGGACCAATGC	45.0	50.0	57.6	58.0	524-642 bp	1.0	85.1
rpl2-rpl23_p4	CGTGTACTCGGGGTAGAAGT	ATCCGTTATGGGACCAATGC	55.0	50.0	58.5	58.0	512-630 bp	1.0	83.9
rpl2-rpl23_p5	CAACGGTGCTGTCCAAAAAT	ATCCGTTATGGGACCAATGC	45.0	50.0	57.5	58.0	590-708 bp	1.0	83.6