Hotspot

rps19-rpl2

LSC · 26149-26761 · 613 bp

Back to Lecanorchis

Pi 0.104

MarkerSeek score 92.60

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: LSC
length_bp: 613
variable_sites: 59
pi: 0.104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 92.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGCCCGAGACCAAGTTACTA	GTTATCCTGCGCTTGGAAGA	50.0	50.0	58.1	58.0	801-947 bp	1.0	88.6
rps19-rpl2_p2	TGCCCGAGACCAAGTTACTA	GCTTGGAAGAAGAAGCAGGA	50.0	50.0	58.1	57.8	791-937 bp	1.0	87.4
rps19-rpl2_p3	GATGCCCGAGACCAAGTTAC	GTTATCCTGCGCTTGGAAGA	55.0	50.0	58.3	58.0	803-949 bp	1.0	86.4
rps19-rpl2_p4	TGCCCGAGACCAAGTTACTA	TGATTCTTCGTCGCCGTAAA	50.0	45.0	58.1	57.6	747-893 bp	1.0	86.4
rps19-rpl2_p5	TGCCCGAGACCAAGTTACTA	TATCCTGCGCTTGGAAGAAG	50.0	50.0	58.1	57.7	799-945 bp	1.0	86.3