Hotspot

rpl20-rps12

LSC · 15688-16679 · 992 bp

Back to Lecanorchis

Pi 0.0384

MarkerSeek score 74.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 992
variable_sites: 37
pi: 0.0384
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GCCCCTATAAAGGTTGAGGC	TGTCACTAAATCTCCTGCGC	55.0	50.0	58.0	58.0	1136-1187 bp	1.0	84.3
rpl20-rps12_p2	GCCCCTATAAAGGTTGAGGC	ACTAAATCTCCTGCGCTTCG	55.0	50.0	58.0	58.1	1132-1183 bp	1.0	83.8
rpl20-rps12_p3	GCCCCTATAAAGGTTGAGGC	CTAAATCTCCTGCGCTTCGA	55.0	50.0	58.0	57.8	1131-1182 bp	1.0	82.7
rpl20-rps12_p4	GCCCCTATAAAGGTTGAGGC	TAAATCTCCTGCGCTTCGAG	55.0	50.0	58.0	57.8	1130-1181 bp	1.0	82.7
rpl20-rps12_p5	TTCTTTTTCGACGTCTCCGG	TGTCACTAAATCTCCTGCGC	50.0	50.0	58.2	58.0	1105-1156 bp	1.0	82.7