Hotspot

trnD-GUC-trnfM-CAU

LSC · 4731-6110 · 1380 bp

Back to Lecanorchis

Pi 0.0933

MarkerSeek score 85.20

Primer available yes

Label trnD-GUC-trnfM-CAU

Feature Metadata

label_name: trnD-GUC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 1380
variable_sites: 126
pi: 0.0933
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnfM-CAU_p1	CGCTCTGACCGATTGAACTA	AAATCCCGGTTTTTCAACGC	50.0	45.0	57.4	57.9	1498-2354 bp	1.0	80.8
trnD-GUC-trnfM-CAU_p2	CGCTCTGACCGATTGAACTA	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.4	57.8	1462-2318 bp	1.0	80.3
trnD-GUC-trnfM-CAU_p3	CGCTCTGACCGATTGAACTA	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.4	57.8	1461-2317 bp	1.0	80.3
trnD-GUC-trnfM-CAU_p4	CGCTCTGACCGATTGAACTA	TTTTCAACGCGGAGTAGAGC	50.0	50.0	57.4	58.6	1488-2344 bp	1.0	77.4
trnD-GUC-trnfM-CAU_p5	CGCTCTGACCGATTGAACTA	GGTTTTTCAACGCGGAGTAG	50.0	50.0	57.4	57.4	1491-2347 bp	1.0	77.3