Hotspot

trnQ-UUG-rps2

LSC · 2223-2786 · 564 bp

Back to Lecanorchis

Pi 0.0412

MarkerSeek score 77.60

Primer available yes

Label trnQ-UUG-rps2

Feature Metadata

label_name: trnQ-UUG-rps2
feature_type: igs
region: LSC
length_bp: 564
variable_sites: 21
pi: 0.0412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-rps2_p1	GACCAAAACCCGTTGCCTTA	AGATTTTTCGATTCCGGCCA	50.0	45.0	58.7	57.9	659-711 bp	1.0	80.0
trnQ-UUG-rps2_p2	ATGAAGACAAAACTGGGGCG	AGATTTTTCGATTCCGGCCA	50.0	45.0	58.8	57.9	705-757 bp	1.0	79.3
trnQ-UUG-rps2_p3	GACCAAAACCCGTTGCCTTA	GCAATTTGTGAGGGTCGTTC	50.0	50.0	58.7	57.7	589-641 bp	1.0	78.6
trnQ-UUG-rps2_p4	GACCAAAACCCGTTGCCTTA	TTGTGAGGGTCGTTCTAGCT	50.0	50.0	58.7	58.4	584-636 bp	1.0	78.4
trnQ-UUG-rps2_p5	ATGAAGACAAAACTGGGGCG	GCAATTTGTGAGGGTCGTTC	50.0	50.0	58.8	57.7	635-687 bp	1.0	77.9