Hotspot

matK-trnQ-UUG

LSC · 1547-2150 · 604 bp

Back to Lecanorchis

Pi 0.112
MarkerSeek score 83.30
Primer available yes
Label matK-trnQ-UUG

Feature Metadata

label_name
matK-trnQ-UUG
feature_type
igs
region
LSC
length_bp
604
variable_sites
66
pi
0.112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnQ-UUG_p1 TTGAAATTCCGACGTTGACG TAAGGCAACGGGTTTTGGTC 45.0 50.0 57.1 58.7 683-2027 bp 1.0 71.9
matK-trnQ-UUG_p2 TTGAAATTCCGACGTTGACG GTAAGGCAACGGGTTTTGGT 45.0 50.0 57.1 59.0 684-2028 bp 1.0 69.6
matK-trnQ-UUG_p3 TTTGAAATTCCGACGTTGACG TAAGGCAACGGGTTTTGGTC 42.9 50.0 57.8 58.7 684-2028 bp 1.0 69.5
matK-trnQ-UUG_p4 TTGAAATTCCGACGTTGACG AAGGTTCGAATCCTTCCGC 45.0 52.6 57.1 57.8 653-1997 bp 1.0 68.2
matK-trnQ-UUG_p5 TTGAAATTCCGACGTTGACG TATAAAGAGATGGGGCGTGG 45.0 50.0 57.1 56.8 711-2055 bp 1.0 67.5