Hotspot

ndhC-trnV-UAC

LSC · 50069-51001 · 933 bp

Back to Lavandula

Pi 0.021

MarkerSeek score 68.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 933
variable_sites: 36
pi: 0.021
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAAAAACA	AGAAGGTCTACGGTTCGAGT	27.3	50.0	52.7	57.8	968-989 bp	1.0	46.9
ndhC-trnV-UAC_p2	TCATATTCGTAAAGCAAAAACA	GAAGGTCTACGGTTCGAGTC	27.3	55.0	52.7	57.5	967-988 bp	1.0	46.9
ndhC-trnV-UAC_p3	AGACTTGATATTATTAGAAATGCCCA	AGAAGGTCTACGGTTCGAGT	30.8	50.0	56.5	57.8	1001-1022 bp	1.0	46.8
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAAAAACA	AAGGCATGTTGGGTCTTTGA	27.3	45.0	52.7	57.6	1035-1056 bp	1.0	46.7
ndhC-trnV-UAC_p5	TCATATTCGTAAAGCAAAAACA	AGGCATGTTGGGTCTTTGAA	27.3	45.0	52.7	57.6	1034-1055 bp	1.0	46.7