Hotspot

ycf3-trnS

LSC · 43842-44615 · 774 bp

Back to Lavandula

Pi 0.021

MarkerSeek score 71.10

Primer available yes

Label ycf3-trnS

Feature Metadata

label_name: ycf3-trnS
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 30
pi: 0.021
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS_p1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	946-1008 bp	1.0	76.7
ycf3-trnS_p2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	945-1007 bp	1.0	76.7
ycf3-trnS_p3	TGCCTCCTTTTCTCCTGAAG	AAGTGAAGGTGCGGAAAGAG	50.0	50.0	57.1	58.1	977-1039 bp	1.0	76.4
ycf3-trnS_p4	GCCTCCTTTTCTCCTGAAGT	AAGTGAAGGTGCGGAAAGAG	50.0	50.0	57.1	58.1	976-1038 bp	1.0	76.4
ycf3-trnS_p5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.1	57.8	917-979 bp	1.0	76.1