Hotspot

trnE-UUC-trnT

LSC · 30159-30752 · 594 bp

Back to Lavandula

Pi 0.0187

MarkerSeek score 68.00

Primer available yes

Label trnE-UUC-trnT

Feature Metadata

label_name: trnE-UUC-trnT
feature_type: igs
region: LSC
length_bp: 594
variable_sites: 20
pi: 0.0187
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	701-735 bp	1.0	84.9
trnE-UUC-trnT_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	680-714 bp	1.0	84.5
trnE-UUC-trnT_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	677-711 bp	1.0	84.3
trnE-UUC-trnT_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	663-697 bp	1.0	84.2
trnE-UUC-trnT_p5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	698-732 bp	1.0	83.7