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atpI-rps2

LSC · 15045-15269 · 225 bp

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Pi 0.0526

MarkerSeek score 78.90

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 22
pi: 0.0526
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	ATGTTGGCCTACTTCCACAC	ATTTCGATTCCAGCGAACGA	50.0	45.0	57.8	58.0	406-408 bp	1.0	83.1
atpI-rps2_p2	ATGTTGGCCTACTTCCACAC	TTTCGATTCCAGCGAACGAT	50.0	45.0	57.8	58.0	405-407 bp	1.0	83.1
atpI-rps2_p3	CGTGTACTTGAAAACCCCCT	ATTTCGATTCCAGCGAACGA	50.0	45.0	57.7	58.0	440-442 bp	1.0	82.2
atpI-rps2_p4	CGTGTACTTGAAAACCCCCT	TTTCGATTCCAGCGAACGAT	50.0	45.0	57.7	58.0	439-441 bp	1.0	82.2
atpI-rps2_p5	ACTTGGCCGTGTACTTGAAA	TTTCGATTCCAGCGAACGAT	45.0	45.0	57.7	58.0	446-448 bp	1.0	81.6