Hotspot

trnS-GCU-trnG-UCC

LSC · 8082-8771 · 690 bp

Back to Lavandula

Pi 0.0252
MarkerSeek score 71.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
690
variable_sites
30
pi
0.0252
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.91
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 730-795 bp 1.0 77.2
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 729-794 bp 1.0 77.2
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 730-795 bp 1.0 77.2
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 729-794 bp 1.0 77.2
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.2 58.2 784-849 bp 1.0 75.9