Hotspot

psbI-trnS-GCU

LSC · 7845-7993 · 149 bp

Back to Lavandula

Pi 0.0336

MarkerSeek score 70.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 149
variable_sites: 8
pi: 0.0336
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATGATCCAGGGCGTAATCCT	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.3	58.0	245-257 bp	1.0	84.4
psbI-trnS-GCU_p2	ATGATCCAGGGCGTAATCCT	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.3	58.0	246-258 bp	1.0	84.4
psbI-trnS-GCU_p3	ATGATCCAGGGCGTAATCCT	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.3	58.1	264-276 bp	1.0	83.2
psbI-trnS-GCU_p4	AATGATCCAGGGCGTAATCC	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.4	58.0	246-258 bp	1.0	81.9
psbI-trnS-GCU_p5	AATGATCCAGGGCGTAATCC	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.4	58.0	247-259 bp	1.0	81.9