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trnH-GUG-psbA

LSC · 75-363 · 289 bp

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Pi 0.0486

MarkerSeek score 82.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 289
variable_sites: 24
pi: 0.0486
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	340-364 bp	1.0	87.8
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	354-378 bp	1.0	87.6
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	353-377 bp	1.0	87.6
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	355-379 bp	1.0	87.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	55.0	50.0	58.1	57.8	392-416 bp	1.0	86.3