Hotspot

syfB

Genome · 159752-161707 · 1956 bp

Back to Laurencia

Pi 0.131

MarkerSeek score 78.00

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 1956
variable_sites: 441
pi: 0.131
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	TGCCACAGAAATATACCGCC	GGGTGTAGCCAAGTGGTAAG	50.0	55.0	58.0	57.9	2780-2788 bp	1.0	87.0
syfB_p2	TGCCACAGAAATATACCGCC	GGTGTAGCCAAGTGGTAAGG	50.0	55.0	58.0	57.9	2779-2787 bp	1.0	87.0
syfB_p3	TGCCACAGAAATATACCGCC	GCTTATTGGGGTGTAGCCAA	50.0	50.0	58.0	57.9	2788-2796 bp	1.0	86.8
syfB_p4	TGCCACAGAAATATACCGCC	ACCACTGATGTAAGCGCAAA	50.0	45.0	58.0	58.1	2423 bp	0.25	48.8
syfB_p5	CAAGTCTGAGGAAAGTCCGG	ACCACTGATGTAAGCGCAAA	55.0	45.0	57.9	58.1	2500 bp	0.25	48.4