Hotspot

orf190

Genome · 71975-72790 · 816 bp

Back to Laurencia

Pi 0.157

MarkerSeek score 79.60

Primer available yes

Label orf190

Feature Metadata

label_name: orf190
feature_type: gene
region: Genome
length_bp: 816
variable_sites: 217
pi: 0.157
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf190_p1	GGAATTGTTTCGGGAAGGGT	TGTCCCCATTTTGGCTCTTT	50.0	45.0	58.1	57.6	1622 bp	0.25	46.2
orf190_p2	GGAATTGTTTCGGGAAGGGT	TTGTCCCCATTTTGGCTCTT	50.0	45.0	58.1	57.6	1623 bp	0.25	46.2
orf190_p3	GTACCAGTACCGCCAACAAT	TGTCCCCATTTTGGCTCTTT	50.0	45.0	57.9	57.6	1478 bp	0.25	46.0
orf190_p4	GTACCAGTACCGCCAACAAT	TTGTCCCCATTTTGGCTCTT	50.0	45.0	57.9	57.6	1479 bp	0.25	46.0
orf190_p5	CCAATTCTGCACCCCATTCT	TGTCCCCATTTTGGCTCTTT	50.0	45.0	58.1	57.6	1588 bp	0.25	45.7