Hotspot

ntcA

Genome · 156811-157485 · 675 bp

Back to Laurencia

Pi 0.109

MarkerSeek score 80.10

Primer available yes

Label ntcA

Feature Metadata

label_name: ntcA
feature_type: gene
region: Genome
length_bp: 675
variable_sites: 128
pi: 0.109
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ntcA_p1	TGCTTCTGCTACAGTTGGTC	TTGTGACTCCATCATACGCG	50.0	50.0	57.8	58.1	1654-1661 bp	1.0	86.0
ntcA_p2	AATGACGCCTGTTACCCAAG	TTGTGACTCCATCATACGCG	50.0	50.0	58.2	58.1	1828 bp	0.25	47.9
ntcA_p3	ACTGCTAAAATGACGCCTGT	TTGTGACTCCATCATACGCG	45.0	50.0	57.8	58.1	1836 bp	0.25	47.9
ntcA_p4	TGCTTCTGCTACAGTTGGTC	CAACGAAGAATCCCTCTGCA	50.0	50.0	57.8	57.9	1067 bp	0.25	47.8
ntcA_p5	TGCTTCTGCTACAGTTGGTC	AACGAAGAATCCCTCTGCAC	50.0	50.0	57.8	57.9	1066 bp	0.25	47.8