Hotspot

moeB

Genome · 152519-153572 · 1054 bp

Back to Laurencia

Pi 0.117

MarkerSeek score 80.20

Primer available yes

Label moeB

Feature Metadata

label_name: moeB
feature_type: gene
region: Genome
length_bp: 1054
variable_sites: 211
pi: 0.117
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
moeB_p1	AAGCTAGCAAGACCGTTTGG	AACAGAAGCTGGAGGAGTCT	50.0	50.0	58.5	58.0	1770 bp	0.25	46.0
moeB_p2	AAGCTAGCAAGACCGTTTGG	ACAGAAGCTGGAGGAGTCTT	50.0	50.0	58.5	58.0	1769 bp	0.25	46.0
moeB_p3	AAGCTAGCAAGACCGTTTGG	ACAATACCCATGCTACGAGC	50.0	50.0	58.5	57.8	1952 bp	0.25	44.3
moeB_p4	AAGCTAGCAAGACCGTTTGG	CTACGAGCAGTACCTTGCAC	50.0	55.0	58.5	58.4	1940 bp	0.25	43.2
moeB_p5	AAGCTAGCAAGACCGTTTGG	ACGCCCTGAAGGATTTGAAC	50.0	50.0	58.5	58.5	1417 bp	0.25	42.5